​Proteins play a crucial role in biological processes, acting as building blocks for cells and performing various functions within organisms. Accurately detecting and analyzing proteins is therefore essential for understanding their structure and function, as well as for diagnosing diseases and developing new drugs. One innovative approach to protein detection is the use of 5CB liquid crystals in conjunction with acoustofluidics. This combination allows for precise manipulation and organization of protein droplets, creating a platform for efficient and sensitive protein detection. By utilizing the unique properties of 5CB liquid crystals and acoustofluidics, we have been able to arrange Liquid crystal droplets in a controlled manner, enabling the detection and analysis of proteins with high sensitivity and accuracy by observing configuration transition in droplets from radial to bipolar configuration.