We have surface re-engineered various proteins (BSA, α-chymotrypsin, α-synuclein) to form solvent-less liquid bio-conjugates with retention of proteins secondary structure that displays soft-solid like properties below 25 °C, but transitions to a liquid phase at higher temperatures. Utilizing this melting transition, we show dissolution of various dry and powdered species of different sizes, and surface chemistries; molecular (angstrom sized) hydrophobic coumarin 153 dye, nanometeric α-chymotrypsin (digestive enzyme), and micrometer sized hydrophilic 1 μm fluorescent polystyrene beads. Our work highlights the ability of these protein-polymer surfactant based solvent-less liquid to dissolve different species that allow it to act as a new universal non-aqueous solvent medium which itself is biologically active to carry out reactions such as proteolytic digestion. Further, we designed a strategy to prepare a low viscous bio-catalytic composite solvent medium by incorporating enzyme (α-Chymotrypsin) polymer surfactant bio-conjugates in neat ionic liquids. The developed composite solvent performed catalytic reactions (limited hydrolysis) when dry and powdered BSA solutes are dissolved in the system which makes them an excellent candidate for biocatalytic reaction platform. Our developed universal solvent matrices are a significant alternative for the current challenges of sustainable solvent research and development.